PJB-2025-1539
Systematic analysis of the bHLH transcription factor family in Toona sinensis: potential regulatory roles in terpene synthesis via the MEP pathway
Liping Ren, Xianhui Deng, Tianhao Hu, Miao Li, Wenyang Wan, Dandan Yin, Zongxin Ma and Xiaohan Cao
Abstract
Basic helix-loop-helix (bHLH) transcription factors which constitute the second-largest transcription factor superfamily in plants, play an important complex regulatory role in the biosynthesis of plant secondary metabolites. However, little information of bHLH family is available in the multi-prupose horticultural plant T. sinensis. Systematic research on the T. sinensis bHLH TF family will enable a comprehensive and in-depth understanding of this species. In this study, we identified 132 TsbHLH family members using phylogenetic analysis and categorized them into 21 subfamilies based on their structural characteristics. Then, the bHLH domain, gene structures, protein conserved motifs, cis-regulatory elements and Chromosomal distribution were probed. In addition, we performed a gene duplication analysis and found the TsbHLH TF family is composed of three gene tandems and 96 segmental duplications, which may be responsible for the prosperity of it. Furthermore, 20 differential expressed TsbHLH genes identified in a T. sinensis transcriptome database were selected to detect their expression across four distinct harvesting periods. Finally, we found that some TsbHLHs may participate in regulating terpene synthesis via the MEP pathway according to the correlation study between the expression of TsbHLHs genes and terpene synthase genes. Our findings provides basic information about TsbHLH TF family and lay a groundwork for further research on the function of bHLH genes in regulating the terpene synthesis
To Cite this article:
Ren, L., X. Deng, T. Hu, M. Li, W. Wan, D. Yin, Z. Ma and X. Cao. 2025. Systematic analysis of the bHLH transcription factor family in Toona sinensis: potential regulatory roles in terpene synthesis via the MEP pathway. Pak. J. Bot., 57(4): DOI: http://dx.doi.org/10.30848/PJB2025-4(40)
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