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First report of Cladosporium cladosporioides instigating leaf spot of Solanum melongena from Pakistan
Abstract
Solanum melongena is a popular and edible vegetable crop of Pakistan. Outbreak of irregular necrotic leaf spots with darker margins was observed. Initially the morphological identification was executed by studying the pathogen. On the bases of olive green colonies, greenish black elongated conidiophores and ellipsoidal branched conidia, the pathogen was identified as Cladosporium cladosporioides. In genetic characterization primers ITS1/ITS4 amplified~650bp DNA fragment of total genomic DNA. BLAST analysis of region (KY290222) exhibited 99% similarity with many strains of C. cladosporioides present in GenBank. The healthy leaves of S. melongena were further exposed to spores of C. clasosporioides to confirm its pathogenicity
