PJB-2018-1799
Pharmacological studies of isolated compounds from Adhatoda vasica and Calotropis procera as an antioxidant and antimicrobial bioactive sources
Waseem Ahmed, Rafia Azmat, Sami Ullah Khan, Shah Masaud Khan, M. Liaquat, Abdul Qayyum and Ayaz Mehmood
Abstract
Adhatoda vasica and Calotropis procera species are worldwide available but poorly investigated as an important resource of bioactive compounds and antimicrobial activities for controlling of many infectious diseases. This article explores the activities of isolated compounds using advanced technology in context of bacterial diseases and suppression of infectious diseases through stictic acid and usnic acid content isolated first time from these species. The estimation of different compounds was carried out in leaves samples of both plants while various compounds like usnic acid, stictic acid and p-hydroxy-benzoic contents isolated through high performance liquid chromatography. The total antioxidant activities of 2, 2’-azino-bis (3- ethylbenzothiazoline-6-sulphonic acid (ABTS) and some active compounds evaluated via different extraction methods such as methanol, ether, acetone and crude extraction with the quantity of these compounds. The Broth microdilution method was performed to determine the minimum inhibitory concentration (MIC) for both species. The obtained data for each parameter was interpreted by applying Complete Randomized Design (CRD) along with factorial arrangements. Mean comparison was performed using LSD test at 5% probability level. The efficiency of extraction of the solvents for the usnic acid, stictic acid and p-hydroxy-benzoic contents found to be methanol > ether > acetone > crude extraction. The MIC values of all extracted compounds showed maximum level of bacterial control. The MIC of methanol extracts of Adhatoda vasica is E-coli E1 (516) μg/mL while Calotropis procera plant’s extract showed higher antimicrobial activity for E-coli E1 511 μg/mL. All bacterial strain showed maximum resistance especially the kidney failure strain E. coli O157:H7. It was concluded that isolation of usnic acid, stictic acid first time from these two species recommended that both species can act as the best resources of antioxidant and antimicrobial agents in controlling of various bacterial diseases
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