PJB-2017-127
ANTIOXIDANT, ANTIGLYCATION AND IMMUNOMODULATORY ACTIVITIES OF SELECTED MEDICINAL PLANTS FROM CENTRAL KARAKORAM NATIONAL PARK (CKNP) GILGIT, PAKISTAN
QAMAR ABBAS, SHER WALI KHAN, MUHAMMAD ISMAIL, SAJJAD ALI AND RAHMATULLAH QURESHI
Abstract
Oxidative species are regularly formed in the physiological system as a result of a number of normal metabolic activities especially the energy related processes. Although there is an innate mechanism of antioxidant defense system but over production of the oxidative species may lead to a worse state formally called “oxidative stress”. The natural endogenous antioxidant defense system is supplemented by phytochemicals with antioxidant potential. The present study was conducted to determine antioxidant, antiglycation and immunomodulatory activities of various solvent based extracts of selected medicinally important plants from Central Karakoram National Park (CKNP), Gilgit, Pakistan. The plants were selected based on their traditional use in different ailments. Among the studied plants, ethyl acetate fraction of Pulsatilla wallichiana showed promising activity against DPPH radicals with its 31µg/mL IC50 which was comparable with the standard antioxidant compound (gallic acid with 23µg/mL IC50). Methanolic fractions of P. wallichiana and Saussurea simpsoniana showed the highest antiglycation activity with 45% and 41%, respectively. The dichloromethane fractions of S. simpsoniana and Salvia nubicola showed highest immunomodulatory activity (51.2 and 54.28 µg/mL IC50, respectively). Among the studied plants, dichloromethane fraction of S. simpsoniana showed highest antiglycation potential (45%), followed by methanolic fraction of P. wallichiana (41%) and S. nubicola (40%). Based on results, it can be concluded that P. wallichiana and S. simpsoniana possess antioxidant, antiglycation and immunomodulatory activities that can be used in drug discovery programme. The mechanistic studies are required to validate the activity of these plants at molecular level.
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