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Variation in Protein Profiles of Phytophthora infestans Isolates Collected from Potato Production Zones of Pakistan.
Abstract
Forty-nine isolates of Phytophthora infestans, the causal organism of late blight in potato, studied to understand variations in protein profile using SDS-PAGE analysis. Of these fifty-five were from potato production zones of Pakistan and four isolates were acquired from Comell University, LJ.S.A. Rye agar medium was used to grow isolates of P. infestans. Polyacrylamide gel was used to compare the protein profile of isolates. Ten bands were found pelymorphic and scored to generate dendrogram. Two major groups (A &: B) were observed with the help of cluster analysis. Group A is furthers divided into six and group B into three subgroups. All the isolates from Comell University and 50% isolates from Pakistan were grouped in the same cluster indicating high level of homology among cach other.
