PJB-1974-11
SIPHON TECHNIQUE FOR ISOLATING BUOYANT SPORES
A.A BHUTTA
Abstract
A siphon technique has been developed to
isolate petrified , spherical, uncompressed, buoyant spores dispersed in soil or embedded in mineral matter. Certain fossil spores and pollen grains can be separated from heavier
mineral matter by suspending them in Thoulet’s
solution (Potassium iodide and Mercuric iodide
: aqueous solution adjusted to sp. Gr.2.5) and in Bromoform 2.3 sp. Gr. As recommended by Naumova and Tiniofeyev in Schopf (1962).
There are some uncompreesed spherical spores and pollen grains which contains air and are, therefore, buoyant. Certain Endogonespores have the same characteristics. In such cases specific gravity methods have been found to be un satisfactory. Petrified , uncompressed spores of Rhynia major Kidston and lang are buoyant and very fragile to handle (Bhutta, 1969). By the siphon technique buoyant spores of Rhynia major have been successfully isolated. The method has been experimented to isolate
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