PJB-2013-297
GENETIC DIVERSITY OF WILD MEDICAGO SATIVA BY SEQUENCE-RELATED AMPLIFIED POLYMORPHISM MARKERS IN XINGJIANG REGION, CHINA
MA JIN-XING1,2 , WANG TIE-MEI1, AND LU XIN-SHI1*
Abstract
SRAP (Sequence-related amplified polymorphism) molecular markers were used to detect the genetic diversity of 22 accessions of wild Medicago sativa from Xinjiang. Studying on the 22 accessions 15 pairs of primer combinations detected a total of 252 bands, and each pair of primers amplified 12 polymorphic bands in average. The percentage of polymorphic bands (PPB) was 78.68%.The Nei’s genetic similarity coefficient of the tested accessions ranged from 0.182 to 0.857 and the average Nei-Li’s coefficient was 0.621. These results suggested that there were rich genetic diversity with the Beijiang resources comparing to Nanjiang resourses. The result from the UPGMA cluster analysis indicated that genetic diversity of wild Medicago could be divided into 5 groups in Xinjiang region and it is of great beneficial to protect alfalfa germplasm resources for developing local husbandry.
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