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PJB-2011-73
MULTISTEP MUTAGENESIS FOR THE OVER-EXPRESSION OF CELLULASE IN HUMICOLA INSOLENS
Abstract
The present study describes the improvement of a thermophilic fungal strain Humicola insolens for cellulase production. H. insolens TAS-13 has the potential to produce 1.00 U/ml/min CMC-ase, 0.43 U/ml/min FP-ase and 0.30 U/ml/min β-glucosidase, which was improved through UV and chemical mutagenesis like N-methyl-N-nitro-N-nitrosoguanidine (MNNG), nitrous acid (HNO2), ethyl methyl sulphonate (EMS) or Ethidium bromide (EtBr). Two alternative mutation steps were carried out and mutants were screened for cellulase production. After first step mutation
