PJB-2010-438
EFFECT OF BACTERIAL CULTURE DENSITY AND ACETOSYRINGONE CONCENTRATION ON AGROBACTERIUM MEDIATED TRANSFORMATION IN WHEAT
HAMID RASHID1, ASMA AFZAL2, M. HAROON KHAN1, ZUBEDA CHAUDHRY3 AND SALMAN A. MALIK2
Abstract
In the present study high efficiency Agrobacterium tumefaciens mediated transformation system of wheat (Triticum aestivum L.) cv. Inqilab-91 was developed by exploiting bacterial culture density and acetosyringone concentration. Agrobacterium strain EHA101 harboring binary vector pIG121Hm, containing gene for GUS activity and hygromycin resistance was used in transformation experiments. Different optical densities (0, 0.25, 0.5, 0.75 and 1.0) of bacterial suspension and different concentrations (0, 50
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