PJB-2010-428
RAPID IN VITRO MULTIPLICATION OF ACACIA NILOTICA SUBSP. HEMISPHERICA, A CRITICALLY ENDANGERED ENDEMIC TAXON
HAIDER ABBAS1*, MUHAMMAD QAISER2 AND BEENA NAQVI3
Abstract
Acacia nilotica Willd. ex Delile subsp. hemispherica is an endangered and endemic taxon reported from Southern Pakistan. Hence an urgent conservation strategy is required due to exposure of the taxon to habitat loss and its over-exploitation. A micropropagation system was developed for Acacia nilotica by comparing MS and B5 media effects on growth. Fresh seeds were collected from the wild, germinated in vitro and these seedlings were used as an explant source. The efficiency of B5 and MS medium was thoroughly examined by augmenting with various levels of BAP along with 0.5 mg/l NAA. MS media proved more appropriate than B5 medium and produced the highest number (4.23) of shoots with 43.2% shoot regeneration frequency in the presence of 2.0 mg/l BAP and 0.5 mg/l NAA. IAA (3.0 mg/l) produced maximum number (2.80) of roots along with the highest rooting frequency (95%).
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