PJB-2010-374
SOLID STATE CULTURING OF THERMOPHILIC FUNGI FOR PHYTASE PRODUCTION
MUHAMMAD MOHSIN JAVED*, WASEEM AHMED, SANA ZAHOOR AND IKRAM-UL-HAQ
Abstract
Seven different types of themophilic fungi such as Aspergillus fumigatus, Humicola insolens, Rhizomucor miehei-I & II, Sporotrichum thermophile, Thermomyces lanuginosus-I & II were isolated from the compost of various localities. Among all isolates, Sporotrichum thermophile was found to be the best isolate for the phytase production. Six different types of solid materials (wheat bran, rice bran, rice husk, fish meal, corn seed and corn gluten) were evaluated as growth substrate for phytase production by Sporotrichum thermophile. Of all the sources tested, wheat bran supplemented with diluent containing (g/L); (NH4)2SO4; 5.0, KH2PO4; 1.0, Yeast extract; 2.0 gave maximum production (4.16 U/mL/min) when 4% volume of the 250 mL conical flask was used after 96 hrs conidial inoculation at 45oC using solid-state fermentation.
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