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HIGH FREQUENCY ADVENTITIOUS PLANT REGENERATION FROM RADICLE EXPLANTS OF AEGLE MARMELOS CORR
Abstract
A procedure was developed which allows high frequency adventitious shoot regeneration from radicle tissues of Aegle marmelos. Adventitious buds were initiated on Murashige & Skoog's (MS) medium containing various combinations of benzyl adenine (BA) and naphthaleneacetic acid (NAA). The medium containing 1.0 mg/1 BA and 0.2 mg/1 NAA produced highest number of shoots per explant with maximum frequency of regeneration. Shoots were elongated by transferring explants with shoot buds to a medium with a low concentration (0.1 mg/1) of BA. The shoots grown in medium containing 25 mg/l indolebutyric acid (IBA) for one week, when transferred to basal medium produced adventitious roots. Maximum rooting (80%) with 3-6 roots per shoot was achieved.
