PJB-2010-62
GENETIC VARIABILITY IN MUTATED POPULATION OF SUGARCANE CLONE NIA-98 THROUGH MOLECULAR MARKERS (RAPD AND TRAP)
IMTIAZ AHMED KHAN, SAJIDA BIBI, SHAFQUAT YASMIN, ABDULLAH KHATRI, NIGHAT SEEMA AND SHAHID AFGHAN*
Abstract
Variability obtained from mutation breeding (gamma rays) was examined through molecular marker techniques (RAPD). A total of 85 loci were amplified, out of which 76.47% were polymorphic and 23.53% were monomorphic. Fragments size ranged from 220bp-2.1kb and fragments produced by various primers ranged from 3-13 with an average of 5 fragments per primer. The highest number of loci (13) was amplified with primer B-07, while the lowest number 3 with primer B-01. Results revealed that mutant P1 (20Gy) contained a specific segment of 2.03kb. Genetically most similar genotypes were P2 (10Gy) and P4 (20Gy) (95.55%) while most dissimilar genotypes were P4 (10Gy) and P3 (20Gy) (63.2%). On the basis of results achieved, the mutants could be divided into four clusters and three groups. Mutants P4 (40 Gy) and P4 (10 Gy) were genetically distinct from other mutants.
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