PJB-2009-222
OPTIMIZED SELECTION AND REGENERATION CONDITIONS FOR AGROBACTERIUM-MEDIATED TRANSFORMATION OF CHICKPEA COTYLEDONARY NODES
M.T. ÖZ, F. EYİDOĞAN1, M. YÜCEL AND H.A. ÖKTEM*
Abstract
An efficient selection system and Agrobacterium mediated transformation for chickpea (Cicer arietinum L.) cotyledonary nodes were investigated. Effect of selective agents and antibiotics on multiple shoot and root induction of cotyledonary nodes and effects of mechanical injury and vacuum infiltration on transformation efficiency were evaluated. Selective agents and antibiotics were applied to explants at different concentrations for one month and numbers of regenerated shoots and roots were recorded. Kanamycin at 100 mg dm-3, hygromycin at 20 mg dm-3, phosphinotricin at 3 mg dm-3 and glyphosate at 5 mg dm-3 were found to be appropriate to select chickpea transformants. Lowest concentrations of all selective agents (50 mg dm-3 kanamycin
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