PJB-2007-268
IN VITRO RE-GENERATION OF GUAVA (PSIDIUM GUAJAVA L.) FROM SHOOT TIPS OF MATURE TREES
ROSHAN ZAMIR, N. ALI*, S.T. SHAH, T. MUHAMMAD AND S.A. SHAH
Abstract
Micropropagation of guava through shoot tip culture from five years old bearing plants was carried out at Nuclear Institute for Food & Agriculture, Peshawar. Shoot tips after sterilization was cultured on modified MS medium supplemented with different concentrations and combinations of BAP and L-glutamin. Maximum number of shoots (72 %) was developed in to plantlets when MS was supplemented with BAP 1 mg/L and 500 mg/L L-glutamine. MS control and BAP 1mg/L combined with glutamine 250 mg/L gave minimum (22%) plantlets. Maximum (3.5) number of shoots per culture was found in 0.5 mg/L BAP alongwith 500 mg/L L-glutamine. MS supplemented with 2.5 mg/L IAA + 2.5 mg/L IBA gave maximum (54) plants rooted with average number of roots (3.8) per plantlet. The In-vitro produced plantlets were successfully established in soil.
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