Paper Details

PJB-2024-610

Genetic diversity in tulip cultivars and Chinese wild species based on SSR markers

Jinhua Tian, Xuejing Ma, Xiuhua Ma, Lianwei Qu, Daocheng Tang and Nan Tang
Abstract

---

Tulip (Tulipa L.) is one of the world-famous ornamental flowers, there are fewer studies on the genetic background of wild tulips and cultivars. The genetic structure, diversity and relationships of 5 tulip species native to China and 105 Dutch tulip cultivars were assessed by 12 highly polymorphic microsatellite markers. The mean values of Shannon’s information index (I), Nei’s diversity index (H) and polymorphism information content (PIC) were 0.91, 0.51 and 0.55, respectively, which indicated the high discriminatory power of the selected markers. One hundred accessions were genotyped using the selected makers and the total observed heterozygosity (Ho) was 0.49. Ho of species was the highest (0.62). Among cultivar groups, Ho of Fosteriana group was the lowest (0.35) while it was highest in Darwin hybrid group (0.55). The genetic distances among species and cultivar groups ranged from 0.0036 to 0.6946. Both the results of Unweighted pair-group method with arithmetic means (UPGMA) and Principal coordinates analysis (PCoA) presented that the 110 materials were separated into three main clusters: species, Fosteriana and T. gesneriana groups. A significant difference could be detected among three clusters (Fst= 0.231, p<0.001), which means 23% of the variance existed among clusters and 77% among species and cultivar groups. In conclusion, the above results indicated that the SSR markers selected in this research can be effectively used for the identification of wild tulips from China and Dutch cultivars. This study thus lays crucial groundwork for the conservation, characterization, and utilization of tulip genetic resources on a global scale

To Cite this article: Tian, J., X. Ma, X. Ma, L. Qu, D. Tang and N. Tang. 2026. Genetic diversity in tulip cultivars and Chinese wild species based on SSR markers. Pak. J. Bot., 58(3): DOI: http://dx.doi.org/10.30848/PJB2026-3(3)  
Download PDF