PJB-1992-13
ESTABLISHMENT OF REGENERATING CALLI AND CELL-SUSPENSION LINE OF BASMATI RICE (ORYZA-SATIVA L CV B.370)
ZAFAR Y; WAJID A; MALIK KA
Abstract
Methods were developed for callus induction and efficient plant regeneration from mature embryos and immature inflorescences of basmati rice (Oryza sativa L cv. Basmati-370). Callusing from seeds of rice was observed in MS or N6 media with various levels of 2,4-D. Somatic embryogenesis was achieved in both media however, response was better in the presence of Kinetin (2 mg l-1). Using field grown immature inflorescences and embryos induction of callus and regeneration was observed. Cell suspension cultures were initiated from 2-3 months old calli of B-370. A fast growing and finely dispersed cell line of Basmati-370 was established. Regeneration of plants was achieved in MS-0 (no hormone).
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