PJB-2024-308
Optimization of fermentation medium and fermentation conditions of biocontrol strain A192 against pepper anthracnose
Xinyue Ma, Mingyang Ma, Xiaoxu Sun, Lingyue Zhu, Wenxing Li, Kangsheng Tian, Yi Zhang, Peiran Wang and Xiaomei Wang
Abstract
Chili anthracnose is one of the three most common and serious diseases in chili peppers. In the early stage of this experiment, a fungal strain A192 was screened from the rhizosphere soil of vegetable crops, which had a good antagonistic effect on Colletotrichum scovillei. It was identified as Penicillium restrictum. To maximize its antifungal effect, the fermentation medium and conditions of strain A192 were optimized. Through single factor experiments, orthogonal experiments, and response surface experiments, the optimal fermentation medium for strain A192 was determined to be dextrin 30.03g/L, diammonium dihydrogen phosphate 3.08g/L, and ZnSO4·7H2O 1.79g/L. Build a model using Design Expert software and predict an inhibition rate of 85.56% under the above conditions. The actual inhibition rate obtained after verification is 85.53%, which is close to the predicted value and increases by 24.24% compared to the initial inhibition rate. Meanwhile, the optimal fermentation conditions are: liquid volume of 100 mL, temperature of 25℃, and fermentation for 10 days.
To Cite this article:
Ma, X., M. Ma, X. Sun, L. Zhu, W. Li, K. Tian, Y. Zhang, P. Wang and X. Wang. 2025. Optimization of fermentation medium and fermentation conditions of biocontrol strain A192 against pepper anthracnose. Pak. J. Bot., 57(5): DOI: http://dx.doi.org/10.30848/PJB2025-5(26)
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