PJB-2024-291
Establishment of tissue culture regeneration system of Gymnotheca chinensis Decne.
Wan-Yun Wu, Shu-Ping Wu and Zhan-Nan Yang
Abstract
In order to establish a simple and efficient tissue culture regeneration system for Gymnotheca chinensis Decne., the tender stems and terminal buds of G. chinensis cultivated in the wild as experimental materials to explore the optimal explant, sterilization conditions of explant, medium for adventitious bud induction, and medium for rooting and subculture in the process of plant tissue culture. The experimental results showed that the top bud as the explant was sterilized by using 0.5 mg/L of sodium penicillin and streptomycin for 3 h+75% ethanol for 30 s+0.1%HgCl2 for 20 min+0.1%HgCl2 for 15 min, with the pollution rate of 22.2% and the browning rate of 33.3%. MS+2 mg/L 6-BA+0.5 mg/L NAA+0.2 g/L activated carbon was the best primary medium with 96.7% bud, MS+1mg/L NAA+0.5 mg/L IBA+0.2 g/L activated carbon as rooting medium, the rooting rate was 66.7%. The best secondary medium was MS+1.0 mg/L NAA+1.0 mg/L IBA+0.2g/L activated carbon with 88.9%, and the survival rate of tissue seedlings reached 100% after transplanting to humus soil 5 d after domestication. This stable tissue culture and regeneration system of G. chinensis, which will provide technical reference for artificial breeding and biological experiments of G. chinensis.
To Cite this article:
Wu, W.Y., S.P. Wu and Z.N. Yang. 2025. Establishment of tissue culture regeneration system of Gymnotheca chinensis Decne. Pak. J. Bot., 57(5): DOI: http://dx.doi.org/10.30848/PJB2025-5(16)
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