PJB-2024-51
Screening and identification of antagonistic fungi of peanut web blotch and their control effects
Yufan Wang, Mingyang Ma, Xinyue Ma, Wenxing Li, Lingle Zhu, Xiaoxu Sun, Xi Zhao and Xiaomei Wang
Abstract
Peanut web blotch is one of the diseases that spread rapidly and cause serious damage in peanut leaf spot diseases. It mainly harms leaves, petioles and stems. In this study, the fungi with obvious antibacterial effect on Didymella arachidicola were screened from the soil. The growth rate method was used for rescreening, and the biocontrol strains with good antibacterial effect were screened and identified. In this study, the bacteriostatic spectrum test was also carried out, and the leaf In vitro culture and greenhouse pot control effect test were carried out with the fermentation broth. The results showed that the F11 strain performed well, the initial screening inhibition zone was 9.56 mm, and the secondary screening inhibition rate was 63.95%. The strain F11 was identified as Talaromyces sayulitensis by morphological identification and molecular biological identification. Through the bacteriostatic spectrum test, it was found that the F11 sterile fermentation broth also had a certain inhibitory effect on other pathogens. The inhibition rates of Exserohilum turcicum and Colletotrichum orbiculare were 83.02% and 81.54%, respectively. The fermentation broth of strain F11 was used to test the biocontrol effect. The results showed that the control effect could reach 87.65%.
To Cite this article:
Wang, Y., M. Ma, X. Ma, W. Li, L. Zhu, X. Sun, X. Zhao and X. Wang. 2025. Screening and identification of antagonistic fungi of peanut web blotch and their control effects. Pak. J. Bot., 57(5): DOI: http://dx.doi.org/10.30848/PJB2025-5(21)
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