PJB-2023-517
Molecular and computational characterization of variation in translation elongation factor methyltransferase efm6 of Sordaria fimicola and its effect on post-translational modifications
Maimoona Ilyas and Muhammad Saleem
Abstract
Post-translational modifications (PTMs) are important alterations of the proteome that make species more compatible with changing environments. The current study was designed to reveal the importance of PTMs and genetic polymorphism of the elongation factor methyltransferase 6 (efm6) in six different strains of Sordaria fimicola through different molecular techniques and computational tools. The efm6 is involved in the methylation of S-adenosyl-L-methionine (SAM) at the N-terminus of the protein which is an important modification for the survival of the species. S. fimicola strains were collected, three from South south-facing slope (S1, S2, S3) and three from North north-facing slope (N5, N6, N7) of Evolution Canyon, Israel. DNA was extracted from all six strains and sequences were obtained after amplification of genes. A total of 56 nucleotide variations in all six strains were observed compared to the reference Sordaria macrospora. There were 7 inter-strain variations observed when strains were compared with each other. Seven inter-strain polymorphic sites were observed in one of SFS strains S3. The predicted putative 3D structure of the EFM6 of S. fimicola revealed that it is consistent with the known structure of SAM. Acetylation sites were predicted at 7 similar positions in both SFS and NFS strains. Glycosylation was predicted on asparagine residues, with N-glycosylation at 5 sites in all strains of S. fimicola while O-glycosylation was predicted on 3 Ser residues in all strains. Potential phosphorylation was determined with 15 sites in all strains respectively. Protein network analysis showed that efm6 has 11 biologically active interactions with other genes that work as a group inferred from S. macrospora. This whole molecular and bioinformatics analysis generated valuable knowledge that stressed environmental conditions are responsible for genetic variations among species and despite inter-species heterogeneity and complexity, the EFM6 is conserved in almost all eukaryotes
To Cite this article:
Ilyas, M. and M. Saleem. 2025. Molecular and computational characterization of variation in translation elongation factor methyltransferase efm6 of Sordaria fimicola and its effect on post-translational modifications. Pak. J. Bot., 57(5): DOI: http://dx.doi.org/10.30848/PJB2025-5(23)
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