PJB-2021-72
In vitro micropropagation of siamese rosewood Dalbergia cochinchinensis Pierre, a timber yielding tree
Supapas Potchara, Chareonsap Piyarat Parinyapong and Poeaim Anurug
Abstract
Dalbergia cochinchinensis, a woody legume, is a high-value timber-yielding tree that is important for both ecological and commercial purposes. To allow efficient In vitro micropropagation, we evaluated the efficiency of plant growth regulators (including kinetin, gibberellic acid, and thidiazuron), different basal media (¼ Murashige and Skoog medium (MS), ½ MS, 1 MS, 2 MS, ¼ woody plant medium (WPM), ½ WPM, 1 WPM, and 2 WPM), gelling agents (phytagel, crystal agar gel G180, food grade agar powder, and bacteriological grade agar powder), and carbon sources (sucrose and glucose) for shoot induction in D. cochinchinensis. Nodal segments, derived from the shoots of 1-year old trees, were surface sterilized and used. Shoots induced by gibberellic acid showed the highest shoot length (17.28 ± 0.20 mm) but were thin, pale green, and displayed shoot tip necrosis. However, shoots induced by kinetin were shorter (15.20 ± 0.41 mm) but were greener and more vigorous. The best conditions for improving shoot induction and elongation were 30 g/L sucrose and 2.6 g/L phytagel. In vitro microshoots were rooted on half-strength WPM augmented with 0.05 mg/L indoleacetic acid. After 1 month of culture, the highest number of roots per shootlet was 6.8 ± 0.58, with an average root length of 54.76 ± 5.22 mm. The plantlets were acclimatized in coconut husks with an 80% survival rate.
To Cite this article:
Potchara, S., C.P. Parinyapong and P. Anurug. 2022. In vitro micropropagation of siamese rosewood Dalbergia cochinchinensis Pierre, a timber yielding tree. Pak. J. Bot., 54(5): DOI: http://dx.doi.org/10.30848/PJB2022-5(36)
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