PJB-2021-1525
Effect of colchicine addition to culture medium on induction of androgenesis in pepper (Capsicum annuum L.)
Nuray Comlekcioglu
Abstract
Anther culture generate completely homozygous line in single generation whereas 6–8 cycles of self-pollination is required to develop homozygous line through conventional breeding method. Experiment on the anther culture in pepper was carried out to determine effect of colchicine on haploid embryo. Semi-solid and double layer culture media were studied. MS (Murashige & Skoog, 1962) medium containing 4 mg l-1 NAA(naphthalene acetic acid), 0.5 mg l-1 BAP (6-benzylaminopurine), 2 g l-1 activated charcoal, 30 g-l sucrose, 10 mg l-1 AgNO3 (silver nitrate) and 0.05 mg l-1 biotin, 0.5 mg l-1 ascorbic acid, 7 g l-1agar was used as control (M1). Semi-solid M2 was % 0.3 colchicine containing M1. The third medium (M3) was double layer. The semi-solid layer was the same of the M1 and liquid layer was % 0.3 colchicine solution. The experiment was carried out in randomized block design with 4 replications. 5 Petri dishes per repetition and 10 anthers per Petri dishes were cultured. Frequency of embryos increased by 89.5% and 36.8% for M2 and M3 medium, respectively, as compared to control (M1).The chromosome doubling of pepper haploids is necessary to restore diploid status and restore fertility. Spontaneous double haploid (SDH) ratios were 33.3% in M1 medium, 57.6% in M2 and 47.3% in M3 medium. Colchicine application significantly affects the number of embryos obtained, embryos rate, the number of regenerated plants and SDH plant numbers.
To Cite this article:
Comlekcioglu, N. 2021. Effect of colchicine addition to culture medium on induction of androgenesis in pepper (Capsicum annuum L.). Pak. J. Bot., 53(3): DOI: http://dx.doi.org/10.30848/PJB2021-3(14)
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