PJB-2022-343
IN-VITRO PHYTOCHEMICAL AND ANTIOXIDANT ACTIVITIES OF CALLUS EXTRACT OF L. USITASSIMUM L.
Keerthi Priya Yadala
Abstract
In this study, callus induction protocol was standardized using different hormone concentrations with Modified Murashige and Skoog medium. The callus was well developed at the hormones concentration of 2,4-dichlorophenoxyacetic acid (0.8 mg/L)+6-benzylaminopurine (1 mg/L).On 30th day the callus culture was used for the experimental study, bioactive compounds like steroids, phytosterols, alkaloids, phenolics, flavonoids and tannins were screened. Total 14 bioactive compounds were identified in L. usitassimum L. extract by FTIR and GC-MS. Among them major compounds observed were n-hexadecanoic acid (12%), pentadecanoic acid ethyl ester (11%) and phytol (10%) followed by other compounds. The callus ethanol extract of L. usitassimum L. was tested against seven bacterial pathogenic organisms. The highest inhibition zone was found to be 21 mm against Shigella boydii. The maximum antioxidant activity (85%) was observed at 500 µg/mL concentration. Further, the callus extract showed better anti-proliferative activity against HepG2 cells 26.4 (μg/mL) and MCF-7 cells 45.1(μg/mL). Upon the results, the callus extract of L. usitassimum L. can be used functional ingredient in health and food applications.
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