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Construction of expression vectors of the melon resistance gene Fom-2 and genetic transformation
Abstract
In this study, we constructed positive and negative expression vectors using the Melon Wilt Resistance Related Gene Fom-2 and transformed the vectors into melon via the pollen tube pathway. In the field, 52 Kan-resistant M4-45 plants and 84 Kan-resistant MR-1 plants were transformed with the Antisense Vector, and the conversion rates were 3.28% and 4.26%, respectively. The PCR results showed that antisense vectors were integrated into the genome of Cucumis melo. The results of a special inoculation test of Fusarium oxysporum melonis showed that the incidence of Fusarium wilt in transgenic plants was 84% and that in non-transgenic plants was 96%. The contents of chitinase, polyphenol oxidase and peroxidase increased with the bacterial treatment time in transgenic plants and non-transgenic plants, and the activities of these three enzymes in transgenic plants were higher than those in non-transgenic plants.
