PJB-2018-1573
QTL analysis for rice seedlings under nitrogen deficiency using chromosomal segment substitution lines
Galal Anis, Yingxin Zhang, Xiaoming Xu, Sajid Fiaz, Weixun Wu, Md Habibur Rahman, Aamir Riaz, Daibo Chen, Xihong Shen, Xiaodeng Zhan, Liyong Cao and Shihua Cheng
Abstract
A population of Chromosomal Segment Substitution Lines (CSSLs) derived from an Indica nitrogen deficiency tolerant Xieqingzao B (XQZB) and an Indica nitrogen deficiency susceptible Zhonghui9308 (ZH9308) was studied. A genetic linkage map was constructed by employing 87 SSR and 33 In Del markers to analyze quantitative trait loci (QTLs) at seedling stage in rice under normal (NN), low (LN) and relative nitrogen (LN/NN) levels. A sum of 9 QTLs was detected in 6 regions on 6 chromosomes, with each QTL explaining 10.58%-27.27% of phenotypic variation. The QTLs controlling shoot dry weight and total dry weight were simultaneously located at RM24718 interval on chromosome 9 under low N conditions. QTLs responsible for root dry weight under LN situations were detected near the marker InD90 on chromosome 6, which explained 16.84% of the phenotypic variation and had a high value for its additive effect (6.14). For future genetic improvement programs, the root dry weight attribute is considered distinctly, which helps to improve, N-deficiency tolerance. This study also providesinsights into fine mapping, gene cloning, and marker assisted selection for future research.
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