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Total phenolics , phenyl alanine ammonia lyase and polyphenol oxidase in in vitro calli of chick-pea

Javed iqbal , shagufta naz , sarwat nazir , faheem aftab and M.S. ahmed

Conditions for in vitro cultures of calli from root , cotyledon and hypocotyl explants of chickpea (cicer ariethinum L. cv CM 72) in M.S. medium were established . total phenolics , phenylalanine ammonia-lyase (PAL) and polyphenol oxidases (PPO) were estimated quantitatively from main callus to 6th sub-culture . the activity of PAL and PPO showed a linear increase upto third sub- culture and then a remarkable decrease from fourth to sixth sub-culture was observed . the data of phenolices was not

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