PJB-2013-67
GENETIC CHARACTERIZATION OF VERTICILLIUM CHLAMYDOSPORIUM ISOLATED FROM PAKISTAN USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) PRIMERS
MUHAMMAD NAZIR UDDIN, SAIFULLAH, MUSHARAF AHMAD, IJAZ ALI, AQIB IQBAL* AND NAQIB ULLAH KHAN
Abstract
This study was undertaken to determine genetic variations, using RAPDs, among Verticillium chlamydosporium isolates infecting Meloidogyne incognita, a major pest in the tomato growing areas of Khyber Pakhtunkhwa province of Pakistan. Significant differences noted among the isolates from different locations and host plants. Maximum loci detected in VC-5 and VC-6, resulting in the highest polymorphism. Among the RAPD Primers, OPB-16 amplified maximum of 10 loci. Monomorphic loci detected only when OPA-19 was used. The bands amplified ranged between 200~2000 bp. The average genetic distance ranged between 16.6 to 54.0%. A dendrogram, constructed on the amplification pattern of these primers, revealed that the isolates could be divided into two different distinct groups. The data further indicated that the genetic differences among the isolates influenced by the geographical location but not by host plant.
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