Paper Details

PJB-2009-212

VIRUS INDEXATION OF IN VITRO REGENERATED SUGARCANE PLANTS

SHAGUFTA NAZ1*, FAYYAZ AHMAD SIDDIQUI2, AAMIR ALI3 AND JAVED IQBAL4
Abstract

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The present study reports the SCMV indexation of In vitro regenerated plants by infectivity assay, serology test and by transmission electron microscopy. The plants were developed by apical meristem, organogenesis and embryogenesis methods. For infectivity test sap of In vitro regenerated plants was inoculated on different cultivars of Sorghum. Out of 166 plants only 44 were SCMV+tive. These plants were further analyzed by using precipitin test. These plants also showed negative result for virus. A total of 17 randomly selected plants i.e.

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