PJB-1995-52
TISSUE CULTURE STUDIES FOR MICROPROPAGATION OF CARNATION (DIANTHUS CARYOPHYLLUS L)
IHSAN ILAHI, FAREEHA AZIZ AND MUSSARAT JABEEN
Abstract
Rapid propagation of carnation (Dianlhus caryophyllus L) was achieved in vitro. Multiple shoots were induced from nodal explants on MS medium supplemented with 1.0, 2.0 or 3.0 mg/l of BAP. For further proliferation the multiple shoots were transferred to MS medium containing different concentrations of BAP and GA3alone or in combination. Maximum number of plantlets per flask were produced on medium enriched with 5.0 mg/l BAP. Rooting was stimulated by subculturing the proliferated shoots on 1/2 MS, 3% sucrose and I % agar. Rooted plantlets were successfully raised in pots filled with soil.
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