PJB-2007-206
COMPARATIVE GROWTH, MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF INDIGENOUS SCLEROTIUM ROLFSII STRAINS ISOLATED FROM DIFFERENT LOCATIONS OF PAKISTAN
DARAKHSHANDA KOKUB1, F. AZAM2*, A. HASSAN2, M. ANSAR2, M. J. ASAD1 AND A. KHANUM1
Abstract
Growth rate of 8 fungal strains of Sclerotium rolfsii on potato dextrose agar plates at 28oC ranged from 0.86-1.35 mm hour-1. Strains D4, D7 and D8 were found to be comparatively fast growing and produced greatest number of sclerotia than others. All strains produced round shaped sclerotia with average diameter of 0.5-2.0 mm. Mycelial compatibility reaction on PDA plates showed that strains D5 and D7 as well as D3 and D8 were compatible with each other. Random amplified polymorphic DNA (RAPD) analysis also revealed that strains D5 and D7 shared 94 % similarity while strains D3 and D8 were 83% similar.
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