PJB-2007-200
INDUCTION AND REGENERATION OF HYPOCOTYL DERIVED CALLI IN HOT CHILLI (CAPSICUM FRUTESCENS L.) VARIETIES
RABBIA HASNAT, NADEEM AKHTAR ABBASI, TOUQEER AHMAD AND ISHFAQ AHMAD HAFIZ
Abstract
An efficient and reliable In vitro protocol was established for induction and regeneration of calli in varieties of hot chilli (Capsicum frutescens L.) var. Nepali and NARC-IV. Early initiation and induction with sustainable calli growth in both varieties was achieved on Murashige & Skoog medium supplemented with 1.5 mg l-1 of 2,4-D. Better performance in both varieties regarding shoot initiation, regeneration rate (%) and number of lateral shoots per regenerants were achieved on medium containing 3.0 mg l-1 BA. Rooting was achieved on half strength MS basal medium containing 1.0 mg l-1 IBA. Plantlets exhibiting normal morphology with extensive rooting were acclimatized in greenhouse.
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