PJB-2025-1548
Strengthening Pisum sativum L. growth through tripartite symbiosis with rhizobia and arbuscular mycorrhizal fungi: a sustainable bio-fertilizer approach
Laila Shaukat, Tabassum Yaseen, Fayaz Asad, Sarir Ahmad, Vasila Sharipova, Fanar Hamad Alshammari and Sajid Ali
Abstract
Growing reliance on chemical fertilizers has reduced soil fertility and damaged the environment, necessitating the development of sustainable options to accelerate agricultural expansion. Arbuscular Mycorrhizal Fungi and Plant Development-Promoting Rhizobacteria (PGPR) provide environmentally friendly solutions by enhancing nutrient uptake and encouraging organic plant development. The purpose of this study is to isolate and describe Rhizobium strains and Arbuscular Mycorrhizal Fungi (AMF) from pea plants in the Charsadda District in order to develop biofertilizers that work effectively for crops and soil development. Using the Yeast-Mannitol Agar Medium (YMA), eight rhizobium strains were isolated and identified from root nodules of Pisum sativum L in ten different areas of Charsadda District. Rhizobium strains actively produced an indole-3-acetic acid (IAA) ranging from 09.48 µgml-1 to 18.84 µgml-1. The qualitative evaluation solubilization of phosphate was determined by the diameter of zones of halo around the colonies on agar medium of Pikovskaya, measuring between 03.30 mm and 08.50 mm. Zinc solubilization was quantified by the diameter zones of halo surrounding the colonies on agar media, measuring between 00.46mm and 04.36mm. All isolates exhibited negative findings in the urease test conducted on Christensen’s Urea Agar medium. AMF spore density and colonization of the root also gave positive results on the ten samples of the survey from the study area. The Sclerocystis species have a maximum concentration of 12.66 gm-100 of other AMF spores, which exhibited 90% of root colonization. Furthermore, all inoculants (PGPR and AMF) had a favorable influence on pea development and growth, as well as a substantial rise in root length of the shoot and dry and fresh pea weight. This was shown by the tripartite symbiosis of the plant Pisum sativum L. with Rhizobia and AMF as a sustainable biofertilizer strategy. A future study should concentrate on optimizing the use of Rhizobium and AMF as biological fertilizers among diverse crops and environmental circumstances to augment their efficacy. Additionally, it is essential to investigate the long-term impacts of these natural fertilizers on soil health and ecosystem sustainability
To Cite this article:
Shaukat, L., T. Yaseen, F. Asad, S. Ahmad, V. Sharipova, F.H. Alshammari and S. Ali. 2025. Strengthening Pisum sativum L. growth through tripartite symbiosis with rhizobia and arbuscular mycorrhizal fungi: a sustainable bio-fertilizer approach. Pak. J. Bot., 57(6): DOI: http://dx.doi.org/10.30848/PJB2025-6(8)
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