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Screening and identification of antagonistic fungi against pepper anthracnose and investigation into its pot-plant control effects
Abstract
In order to enrich the high-quality biocontrol fungi resources for pepper anthracnose, this experiment obtained 282 fungal strains isolated and purified from soil by plate coating method and screened 7 antagonistic fungi with obvious inhibitory strips by using the plate standoff method and re-screened the inhibitory effect of the fungal fermentation broth by using the method of fungal plate containing poisonous medium. The results showed that the fungal strain A192 had a better inhibitory effect, and the inhibition rate of the rescreened fermentation broth was 61.29%. Morphological identification and molecular biological identification of the fungal strain identified as Penicillium restrictum. The fermentation broth of antagonistic fungal strain A192 has a certain degree of inhibitory effect on 13 pathogenic fungi. It has inhibition rates of 80.18%, 79.11%, 77.35%, and 71.67% against Sclerotinia sclerotiorum, Fusarium oxysporum, Alternaria lternata, Phoma arachidicola, respectively. A pot plant control effect test was conducted using the fermentation solution of fungal strain A192, and the control effect was 85.13%.
