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Plant regeneration from excised cotyledon of Aegle marmelos Corr
Abstract
A protocol for excising and culturing cotyledon explants from seeds of different ages of Aegle marmelos was developed. Cotyledon explants formed callus and shoot buds on agarsolidified Murashige & Skoog (MS) medium containing several combinations of 6-benzylaminopurine (BAP), indole-3-acetic acid (IAA) and gibberellic acid (GA3). The highest frequency of adventitious bud forming explants and maximum number of shoots per explant were obtained from 110-150 days old cotyledons. BAP with lAA or GA3gave better result than BAP alone. Shoots were elongated by transferring explants with shoot buds to same basal medium containing 1.0 mg/I kinetin (kn) and 0.1 mg/I lAA. The in vitro regenerated shoots rooted when cultured on half strength MS medium containing 25 mg/I indole-3-butyric acid (IBA). Regeneration by this method may be useful for mass propagation of A. marmelos.
