PJB-2024-1507
High-throughput transcriptome sequencing and analysis of the endangered anticancer medicinal plant Sinopodophyllum hexandrum (Royle) T. S. Ying
Haona Gao, Zheng Zhang, Lu Li, Xiufang Zhao and Wei Liu
Abstract
Sinopodophyllum hexandrum (Royle) T. S. Ying is a traditional medicinal plant in China. Podophyllotoxin, a chemical compound contained in its rhizomes, has important anticancer medicinal value for the treatment of cervical cancer, metrocarcinoma, leukemia, and rheumatism. To obtain the information characteristics of the transcriptome of S. hexandrum, the Illumina HiSeqTM2000 sequencing system was used as the library sequencing platform. The non-reference transcriptome sequencing analysis of the roots, stems and leaves of S. hexandrum was carried out by double-end sequencing method, and 74026 unigenes with high reliability of annotation were obtained. The COG and GO functional classification of unigenes in the transcriptome of S. hexandrum showed that the proportion of unigenes associated with metabolic process, catalytic reaction function, and binding function accounted for a larger proportion. The results of the KEGG analysis showed that S. hexandrum transcriptome unigenes were annotated for 125 metabolic pathways. The number of unigenes annotated to the metabolic pathway was the highest, up to 2921 (24.96%), and the pathway ID of this pathway was ko01100; followed by the biosynthetic pathway controlling secondary metabolites, with 1425 (12.18%) annotated genes, corresponding to the pathway ID of ko01110; in third place was the pathway controlling ribosome metabolism, with 889 (7.6%) annotated genes, corresponding to the pathway ID of ko03010. High-throughput transcriptome sequencing is the sequencing method selected in this study. Subsequently, the overall transcription of S. hexandrum was explored by bioinformatics methods such as COG, GO, and KEGG. These results provide theoretical support for the analysis of the S. hexandrum biosynthesis pathway, the mining of key regulatory genes of podophyllotoxin, and the innovative development and utilization of S. hexandrum resources
To Cite this article:
Gao, H., Z. Zhang, L. Li, X. Zhao and W. Liu. 2024. High-throughput transcriptome sequencing and analysis of the endangered anticancer medicinal plant Sinopodophyllum hexandrum (Royle) T. S. Ying. Pak. J. Bot., 56(1): DOI: http://dx.doi.org/10.30848/PJB2024-1(26)
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