Paper Details


Evaluation of green synthesized silver nanoparticles against bacterial pathogenic strains of plants

Muhammad Iqbal, Naveed Iqbal Raja, Said Akbar Khan, Aamir Ali, Asma Hanif, Mubashir Hussain, Tauseef Anwar, Huma Qureshi, Maimona Saeed, Abdul Rauf, Sulaiman Ali Alharbi And Mohammad Javed Ansari and Rana Khalid Iqbal

Aqueous leaf extract of Hyppophae rhamnoides leaves was used to create silver nanoparticles as a reducing and capping agent. Techniques like the UV-Vis spectrophotometer, scanning electron microscopy (SEM), X-ray diffraction (XRD) analysis, and fourier transform infrared spectroscopy (FTIR) analysis were used to characterize the synthesized nanoparticles. Transmission electron microscopy (TEM) provides size distribution specifying sizes of silver nanoparticles ranging from 13 nm to 34 nm in diameter. The shape of the nanoparticles was amorphous, ranging from spherical to rectangular. The absorption bands at 2526, 2340, 2159 and 2030 cm−1 are related to the carbonyl group (C=O), confirming more biomolecule involvement with C=O functional groups. The peaks 1869, 1845 and 1829 cm−1 are associated with aldehyde (C=O) and alkenes (C=C) functional groups containing compounds. The peak at 668 cm−1 depicted the presence of an aromatic ring. Phytochemical profiling of green synthesized AgNPs revealed the incidence of phenols, terpenoids, flavonoids, and phytosterols being involved in successfully reducing and capping nanoparticles. Maximum antibacterial activity was found in higher concentrations of AgNPs (0.35mg/100ul) against Ralstonia solanacearum and Pseudomonas syringae. Green synthesis is fast, cost-effective, non-hazardous, and environment-friendly compared to its chemical synthesis counterpart. AgNPs can be used as a source of antibiotics in the future

To Cite this article: Iqbal, M., N.I. Raja, S.A. Khan, A. Ali, A. Hanif, M. Hussain, T. Anwar, H. Qureshi, M. Saeed, A. Rauf, S.A. Alharbi and M.J. Ansari and R.K. Iqbal. 2023. Evaluation of green synthesized silver nanoparticles against bacterial pathogenic strains of plants. Pak. J. Bot., 55(5): DOI:  

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