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Response surface methodology for enhanced CMcase and biomass production by bacillus licheniformis TLW-3

Tabbassum Kiran, Wajeeha Asad, Munazza Ajaz, Farah Saleem and Shiekh Ajaz Rasool

Bacillus licheniformis recognized as safe and has unique genetic characteristics that make it important for industrial enzyme production. cellulase enzyme demand has increased globally due to its various applications in different industrial setups. The current era of cellulase application is the bioconversion of agro-industrial wastes into valuable products, which are then fermented in to ethanol. Previously, the OVAT (one variable at a time) approach was used, but this method is unable to provide information regarding interaction effects from different factors. Response surface method (RSM) is provides a solution to study the interaction of multiple parameters using a small number of experiments. During projected research, the central composite design (CCD) was designed with Minitab software (version17) using six factors (concentration of carboxymethyl cellulose (CMC), peptone, and yeast extract, incubation time, pH, and temperature) that were determined by OVAT. A total of 53 experiments were designed and conducted. Results analysis was done in the same software. The obtained model was found to be good fit with non significant error, which indicates the model’s fitness. According to the response and contour plots, good interactions were found among the studied factors. Finally, biomass production was found to be 110g/ml and enzyme yield was increased up to 640.86IU/ml/min using the RSM

To Cite this article: Kiran, T., W. Asad, M. Ajaz, F. Saleem and S.A. Rasool. 2024.  Response surface methodology for enhanced CMcase and biomass production by bacillus licheniformis TLW-3. Pak. J. Bot., 56(4): DOI:  

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