PJB-2022-1534
The perennial sweet sorghum cultivar and its recruiting rhizosphere dominant bacterial taxa under field growth
Gui Hua Lu, Zhiye Na, Zijian Zhang, Rui Cao, Aliya Fazal, Shoucheng Huang, Hanyue Zhang, Jialing Chen, Rui Cheng, Yan Li, Yonghua Yang, Hongjun Yang and Zhong Yuan Na
Abstract
Breeding new perennial sorghum germplasms has been ongoing for decades since perennial crops provide significantly greater environmental benefits in terms of resource utilization, soil erosion, and soil conservation. However, perennial sweet sorghum cultivars or varieties have rarely been reported to date. Additionally, little is known about the potential consequences of perennial sorghum on belowground soil microbial communities. In this study, we reported the perennial sweet sorghum cultivar NaPBS778 (simply N778) with prominent panicle length, plant height, stalk weight, and Brix, which was generated from an annual sweet sorghum cultivar via the Gene Phenotype Induction Technique (GPIT). Further, we designed an experiment to detect the dominant rhizosphere bacterial taxa of the perennial sweet sorghum cultivar N778 and its control sorghum line TP60 at the flowering and maturation stages under natural field growth conditions. The Illumina MiSeq platform was utilized to high-throughput sequence amplicons of the V3–V4 hypervariable region of the 16S rRNA gene (16S rDNA). According to our findings, almost all six alpha diversity indices of N778 rhizosphere soil from either aerial roots and primary roots showed no significant difference when compared to those of TP60 at the same growth stage. There were also no significant differences in the beta diversity of rhizosphere soil between N778 and its control TP60 at the flowering stage. at the maturation stage; however, significant differences in taxonomic and phylogenetic beta diversity of rhizosphere soil were found between N778 and its control TP60. Furthermore, at the maturation stage, the relative abundances of two genera, Pseudarthrobacter and Pseudomonas, were significantly higher in the rhizosphere soil of N778 than in all other groups. Our results indicate that two dominant OTUs belonging to Pseudarthrobacter and Pseudomonas with stress tolerant phenotypes are significantly enriched in the rhizosphere of N778 at the maturation stage, implying that the perennial sweet sorghum cultivar N778 could recruit potential psychrotolerant bacterial taxa into its rhizosphere and assist it in surviving the winter below freezing temperature
To Cite this article:
Lu, G.H., Z. Na, Z. Zhang, R. Cao, A. Fazal, S. Huang, H. Zhang, J. Chen, R. Cheng, Y. Li, Y. Yang, H. Yang and Z.Y. Na. 2022. The perennial sweet sorghum cultivar and its recruiting rhizosphere dominant bacterial taxa under field growth. Pak. J. Bot., 54(3): DOI: http://dx.doi.org/10.30848/PJB2022-3(46)
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