PJB-2021-303
An efficient and improved micropropagation method of Rumex nervosus: a valuable medicinal plant from Saudi Arabia
Norah S. Alfarraj, Fahad Al Qurainy, Mohammad Nadeem, Mohamed Tarroum, Saleh Alansi, Abdalrhaman M. Salih, Norah Alenezi, Hassan O. Shaikhaldein, Asma R. Alanazi and Latifah A. Al-Humaid
Abstract
Rumex nervosus, a large annual herb is a member of the Polygonaceae family, It is an edible medicinal plant that shows antimicrobial and antioxidant activities and used in traditional medicine to treat various ailments. In this study, a new and improved in vitro propagation protocol was developed for R. nervosus. After 1 week of inoculation on plant growth regulator-free Gamborg's B-5 basal (B5) medium, significant seed germination reached 90%. The highest shoot multiplication rate was observed on B5 medium supplemented with 0.5-µM benzylaminopurine (BAP); 7.8 shoots per explant). The maximum mean number of roots was obtained on B5 medium with 0.5-µM 1-naphthaleneacetic acid (maximum number of roots: 29; mean value of root length: 5 cm). Plants were successfully acclimatized into plastic pots containing sterile soil and sand at a 1:1 ratio. Callus induction was best supported on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2.5 µM) and BAP (0.1 µM).
To Cite this article:
Alfarraj, N.S., F. Al‑Qurainy, M. Nadeem, M. Tarroum, S. Alansi, A.M. Salih, N. Alenezi, H.O. Shaikhaldein, A.R. Alanazi and L.A. Al-Humaid. 2022. An efficient and improved micropropagation method of Rumex nervosus: a valuable medicinal plant from Saudi Arabia. Pak. J. Bot., 54(5): DOI: http://dx.doi.org/10.30848/PJB2022-5(44)
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