PJB-2021-94
Molecular characterization of Botryosphaeriaceae causing stem end rot of mango in Pakistan
Sumera Abrar, Shazia Iram, Barira Bilal, Amna Butt and Rabia Shabbir
Abstract
In this study, molecular, morphological and pathological characterization of postharvest fungal pathogens associated with stem end rot of mango fruit was done. A systematic survey was conducted in the farmer (uncontrolled practices) and demo blocks (controlled practices) of 6 districts of Punjab and Sindh for the disease assessment of collected 25-30 samples of mangoes. The stem end rot disease was found 100% prevalent in all the locations while disease incidence was high in farmer blocks. DNA was isolated from the stem end rot isolated fungal strains and the amplified products from ITS-5.8S-rDNA were sequenced which showed 96-100% similarity with previously identified sequences in Genbank. As a result of molecular sequencing analysis, two species of Botryosphaeriaceae, Lasiodiplodia theobromae and Botryosphaeria dothidea were detected. The pathogenicity tests were applied to determine the aggressiveness of postharvest fungal pathogens by plug placement method and found high toxicity on mango twigs and potato tubers but detached mango leaves showed no lesions. Results obtained through this work would be supportive to develop the management policies to reduce the afflictions in orchards caused by mango stem end rot.
To Cite this article:
Abrar, S., S. Iram, B. Bilal, A. Butt and R. Shabbir. 2022. Molecular characterization of Botryosphaeriaceae causing stem end rot of mango in Pakistan. Pak. J. Bot., 54(5): DOI: http://dx.doi.org/10.30848/PJB2022-5(18)
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