PJB-2020-149
EFFECT OF IONIZATION RADIATION ON MORPHOLOGICAL, BIOCHEMICAL AND GENETIC ASPECTS OF Zea mays (L.) AND Cucumis sativus (L.)
Amna Munawar
Abstract
The present study was designed to assess the germination of seeds, morphological, physiological, biochemical and genetic changes in Zea mays (L.) as well as Cucumis sativus (L.) after exposure to gamma radiation. Zea mays is an significant cash crop of world. Cucumis sativus is an important fruit of Pakistan. Under study plants showed significant results in. germination, morphology and biochemical which correlate with the change in genetic pattern after exposure different doses of gamma radiation (100, 200, 300, 400, 500, 600, 700, 800, 900 and 1000 Gy). Seed germination was assessed by final percentage germination (FPG), germination energy (GE), and germination speed (GS).Results showed that the FPG, GS and GE at 600 Gy showed good results in both plants while other doses reduce the germination.1000 Gy dose completely destroy the genes for germination. The morphological parameters studied by leaf length (LL), root length (RL), plant height (PH), number of leaves (NL), and total standing biomass (TSB) The dose of 600 Gy effective for LL, RL, NL and in TS but not effective as compared to control in both plants. Chlorophyll content assessment showed that 600Gy dose increases the chlorophyll a and b as well as over-all chlorophyll substance. The Peroxidase activity (mg/ml) in terms of optical density at 470 nm was also determined showed that peroxidase activity increased by increasing the radiation dose. Lipid peroxidation was analyzed in relation to MDA Equivalent (nano molar per milliliter). MDA content increased in both plant by increasing the dose. The genetic variation was analyzed by DNA amplification, results showed that DNA fragmentation increased as the dose was increased in both plant. The intensity rays inhibited the seed germination and plant growth. The Peroxidase and lipid peroxidation activity showed that these plants under oxidative stress which is lethal for both plants. It is recommended that save the seed of plant from exposures of gamma radiations.
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