PJB-2020-133
A NEW HERBAL COMBINATION TARGETS INSULIN RESISTANCE GENES AND ADIPOKINES IN TYPE 2 DIABETIC RAT MODEL
Sana Eijaz
Abstract
Some nutritionals herbs are well known for their anti-diabetic activity. However, adequate data is not available regarding their exact mechanism of action. In this study, we used a new combination of Trigonella foenum graceum (Tfg), Cinnamomum cassia (Cc), Momordica charantia (Mc), Eugenia jambolana (Ej), Gymnema sylvestre (Gs) and Camellia Sinensis (Cs) and investigated their effect on the blood glucose level, body weight, adipokines level and expression of certain genes in the insulin signaling pathway in adipose and skeletal muscle tissues in type 2 diabetic rat models. Wister rats were used to develop type 2 diabetes using high fat diet and streptozotocin and confirmed with oral glucose tolerance test (OGTT). After administration of the combination extract, glucose level and body weight were monitored throughout the study. The expression of insulin signaling genes was analyzed in skeletal muscle and adipose tissue by RT-PCR adipokines level was measured by ELISA. Moreover, the level of adipokines was also measured by using ELISA. Significant improvement was observed in the blood glucose level and body weight of diabetic rats that received the herbal combination extract. The expression levels of several genes including insulin receptor (INSR), insulin receptor substrate-1 (IRS-1), phosphoinositide 3-kinase (PI3-K), protein kinase B (PKB), and phosphoinositide-dependent protein kinase-1 (PDK-1) were significantly increased whereas those of protein tyrosine phosphatase-1B (PTP-1B) and protein kinase C-theta (PKC-θ) were significantly decreased in both skeletal muscle and adipose tissue of the test group as compared to the diseased control. Moreover, herbal combination extract restored the level of serum adiponectin and leptin. Our data suggests that these herbs when given in combination may exert their anti-diabetic effect due to their action on insulin signaling genes and adipokines level. These herbs may offer a safe and economic strategy and has nutritional significance for the cure and prevention of type 2 diabetes.
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