PJB-2020-39
ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF Salacca zalacca (GAERTN.) PEEL EXTRACT ON FIBROBLAST CELLS-INDUCED PB
Ermi Girsang
Abstract
Background : Salacca zalacca or snake fruit is an indigenous plant in Indonesia that can be separated into peel, flesh and seed. The peel of S. zalacca are usually discarded as waste, meanwhile it exhibits antioxidant and anti-inflammatory activities. Objective: We investigated the antioxidant, anti-inflammatory, anti-necrosis effects of S. zalacca peel extract (SPE) on Pb-induced fibroblast cells. Materials and Methods: SOD was measured as antioxidant activity, necrotic, live cells using flow-cytometer, while COX-2, COL-3 gene expression using RTPCR of Pb-induced fibroblast cells treated with SPE. Results : SPE increased the SOD activity, SPE 25 µg/ml had the highest SOD (13.46 U/ml) compared to other treatments. SPE 100, 25 µg/ml was similar to decrease the necrotic cells and increase live cells. SPE decreased COX-2 gene expression, SPE 25 µg/ml was the most active to decrease COX-2 gene expression also increased COL-3 genes expression on Pb intoxication cells model. Conclusion: The present study reveals that SPE has antioxidant and anti-inflammatory potency, decrease necrotic cells, and increase COL-3.
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