PJB-2020-29
FUNGICIDAL ACTIVITY OF ORGANIC SOLVENT EXTRACTS OF CINNAMON BARK AGAINST MACROPHOMINA PHASEOLINA
Maliha Uroos
Abstract
Antifungal activity of bark extracts of cinnamon (Cinnamomum verum J. Presl) was checked against Macrophomina phaseolina, a widely occurring highly devastative fungal pathogen that inhibits in soil and seeds. Bark was extracted in n-hexane, chloroform, ethyl acetate and methanol. Malt extract broth was served as a medium for pathogen growth, 7 concentrations ranging from 3.125 to 200 mg mL-1 were prepared. Antifungal effect of these extracts was studied in vitro. All the extracts suppressed the fungal growth to variable extents. Chloroform extract demonstrated the maximum antifungal potential followed by ethyl acetate and n-hexane resulting in 18–50%, 13–37% and 4–39% suppression of fungal growth in comparison of control treatment. GC-MS of chloroform extract exhibited the occurrence of six compounds with 2-methoxycinnamic acid as the most abundant compound in the extract with 63.09% of the total. Other common compounds were 1,2-benzenedicarboxylic acid, mono(2-ethylhexyl) ester (20.29%) and 2-methoxycinnamaldehyde (12.25%). Keywords: Antifungal, cinnamon, GC-MS, organic solvent extracts.
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