PJB-2019-1018
EFFECT OF PRETREATMENT OF BACILLUS SUBTILIS BIOMASS ON BIOSORPTION AND ITS REAL TIME APPLICATION
Vinay kumar Chintalapudi
Abstract
The research study investigated the biosorption behavior of Pb (II) ions by treated and untreated biomass of B. subtilis. At initial biosorption conditions the biosorption efficiency was found to be 36.75%. Various biosorption parameters like temperature, biosorbent dosage, agitation speed, pH and contact time was optimized for both control and treated biomass of B. subtilis. At the optimized conditions of the control biomass which showed maximum biosorption efficiency of 58.04%, the biomass was treated with different chemicals. The biomass treated with formaldehyde showed highest efficiency of 80.9%. The biosorption efficiency of treated biomass was further enhanced by optimization and showed maximum efficiency of 89.8% for Pb (II) ions. SEM (Scanning Electron Microscope) and EDX (Energy dispersive X- ray) analysis evaluates the structural and elemental changes occurred as a result of biosorption. Functional groups that are involved in biosorption were revealed by FTIR (Fourier Transform Infrared spectroscopy). Kinetic data showed the best fit with pseudo second order model. Effective removal of lead ions from industrial contaminated water sources by pretreatment biomass of B. subtilis elucidates its potential use as biosorbent for remediation of metal ions.
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