PJB-2019-964
EVALUATION OF RESISTANT CULTIVARS AGAINST CITRUS TRISTEZA CLOSTERO VIRUS
MUHAMMAD BILAL AMIR
Abstract
Citrus Tristeza disease is the emerging devastating threat of citrus plants worldwide particularly in Punjab and KPK provinces of Pakistan. To evaluate the resistant cultivars, research conducted in the Serological laboratory of Plant Virology Section, Plant Pathology Research Institute Faisalabad, Punjab, Pakistan. Fourteen hundred leaf samples of 14 citrus cultivars collected from Citrus Research Institute Sargodha, Pak. For the detection of tristeza disease, leaf sap tested through a Double Antibody Sandwich Enzyme-linked Immunosorbent Assay technique. Dilution of coating antibody and antibody-enzymes conjugate, preparation of washing and extraction buffer, and substrate buffer by adding p-Nitro phenyl Phosphate (pNP) by following the descriptions of ELISA-kit. Reaction observed by yellow color development. The intensity of color development is directly proportional to the amount of pathogen enzyme trapped. Among all the cultivars highest infection found in the Gil-Gil Citrus maxima i.e., 24% and lowest in seedless kinnow C. reticulata i.e., 3%. Seedless kinnow has a maximum level of resistance against Citrus tristeza Clostero virus disease.
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