PJB-2019-953
ASSESSMENT OF ANTIBACTERIAL AND RADICAL SCAVENGING ACTIVITIES OF PHENOLIC COMPOUNDS EXTRACTED FROM ZIZIPHUS MAURITIANA (LAM)
Robina Liaqat
Abstract
Current study is based on the extraction of free and bound phenolic acids from leaves, stem, and flowers of Ziziphus mauritiana plant comparing two extraction procedures followed by antimicrobial activity against two bacteria, i.e. Escherichia coli and Staphylococcus aureus. HPLC profiling elucidates the existence of 17 phenolic compounds, consisting of 10 phenolic acids and 7 flavonoids in which 3 flavonoids were quantified such as naringin, rutin and naringenin and 4 flavonoids were unknown which were identified but not quantified. It was observed that bound phenolic acids possess greater fraction than free phenolic acids. The results from ultrasonic base hydrolysis revealed the p-coumaric, ferulic, 4-hydroxy-3,5-dimethoxybenzoic, 3,4-dihydroxy-cinnamic and sinapinic acids while sonication extraction method show vanillate, sinapinic, 4-hydroxy-3,5-dimethoxybenzoic and chlorogenic acids as main constituents in leaves, stem and flowers respectively. Furthermore, radical scavenging activity was found greater in leaves and stem due to higher amount of total phenolics content, while total flavonoids are higher in flowers and total tannins in leaves. Z. mauritiana leaves, stem and flowers extracts observed to be very efficient against E. coli and S. aureus.
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