PJB-2019-869
Functional characterization of a potent antimicrobial and insecticidal chitin binding protein from seeds of Iberis umbellata L.
Ahsan Saeed, Zahra Rafiq, Qamar Saeed, Binish Khaliq, Anwar Ullah, Sohaib Mehmood, Zahid Ali, Muhammad Yasin Ashraf and Ahmed Akrem
Abstract
Chitin-binding proteins belong to the 2S albumin family and are helped in the plant defense, especially against fungal pathogens. A chitin-binding protein, Iu-CBP, has been identified and characterized from Iberis umbellata seeds. The purified form of this protein showed approximately an 11 kDa band under non-reduced condition on SDS-PAGE. LC-MS/MS provided a single fragment of amino acid sequence of 23 residues (QAVQSAQQQQGQVGPQQVGHMYR). UniProtKB database showed 100% sequence similarity with Moringa oleifera chitin-binding protein (Mo-CBP3-1) which classically contained two proteolytically matured α-helical chains linked by disulfide bonds along surfaced Arginines responsible for antimicrobial activity. Iu-CBP showed antimicrobial activity against bacterial pathogens i.e Bacillus subtilis, Xanthomonas oryzae, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa at concentrations of 5.0 and 10.0 µg per disc. Similarly, a 20 µg/disc dose of Iu-CBP inhibited the mycelial growth of Aspergillus flavus. At a concentration of 3.0 mg/ml, Iu-CBP had a significant reduction in progeny population of Sitophilus oryzae and Tribolium castaneum. Chitin-binding proteins have historically been studied as a source of carbon and nitrogen in plants; more recently, their thermostability, antimicrobial and insecticidal properties, along with their water sterilizing qualities have attracted the interest of the scientific community.
To Cite this article:
Saeed, A., Z. Rafiq, Q. Saeed, B. Khaliq, A. Ullah, S. Mehmood, Z. Ali, M.Y. Ashraf and A. Akrem. 2021. Functional characterization of a potent antimicrobial and insecticidal chitin binding protein from seeds of Iberis umbellata L. Pak. J. Bot., 53(4): DOI: http://dx.doi.org/10.30848/PJB2021-4(16)
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