PJB-2019-846
Insilico analysis and profiling of miRNAs of biofuel plants
Izwa Islam
Abstract
MicroRNAs are the non-coding RNAs consisting of nucleotides ranging from 18-22. They are involved in gene silencing and RNA interference in plants and animals respectively. In recent study the sequences of microRNAs of Zea mays were taken from miRBase and then subjected to blast to get their similarity with three other biofuel plants including Oryza sativa, Panicum hallii, Brachypodium distachyon. Most of the times their waste material is used for the biofuel production. Clustal-W was then used to find the conserved regions among he genome of all these four plants. Then their secondary structures were profiled which helps in better understanding of biofuel properties at genetic level. This study helped in finding the conserved sequences of microRNAs among various plants so that a single genetic manipulation can enhance biofuel properties in all of them. In future, its practical approach can be achieved as well as other microRNAs can also be identified by using ESTs for the same purpose.
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